Study Guide 4-5

Lecture 4.5 Competencies and Study Guide

Krebs Cycle, ETC and OXPHOS

Vocabulary

  • Pyruvate oxidation
  • Krebs cycle

Competencies

  1. Describe the discovery of William Harvey.
  2. Describe the experiments and discovery of Joseph Priestly.
  3. Explain how those discoveries are related to respiration and metabolism.
  4. Explain why glycolysis cannot explain why cells use oxygen and produce carbon dioxide.
  5. Describe what happens to cells’ use of oxygen and production rate of carbon dioxide when fed certain organic acids.
  6. Describe the biochemical pathway from succinate to oxaloacetate.
  7. Explain why one would predict that feeding cells oxaloacetate would increase oxygen consumption and carbon dioxide production rates.
  8. Describe the biochemical pathway from citrate to succinate.
  9. Describe the basic experimental approach used by Adolf Krebs.
  10. Explain Krebs and Johnson’s (1937) hypothesis.
  11. Predict the effect malonate would have on CO2 production if the Krebs and Johnson hypothesis were true.
  12. Describe the results of Krebs and Eggleston (1940) and explain how whether they support, contradict or say nothing about the Krebs and Johnson hypothesis.
  13. Describe the biochemical pathway from pyruvate to oxaloacetate.
  14. Describe where in a eukaryotic cell glycolysis, pyruvate oxidation and the Krebs cycle occur.
  15. Describe and explain, in as much detail as you can, pyruvate oxidation and the Krebs cycle.